The isolation of Nitrosomonas europaea in pure culture.
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چکیده
منابع مشابه
Mutagenesis of hydroxylamine oxidoreductase in Nitrosomonas europaea by transformation and recombination.
Mutagenesis of Nitrosomonas europaea was achieved by electroporation and recombination. To demonstrate this, an aminoglycoside 3'-phosphotransferase (kan) gene was specifically inserted into each of the three gene copies of hao individually. Southern hybridizations and PCR analysis showed the incorporation of the kan gene at the chosen genetic loci. The isolation of mutant strains was achieved ...
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Although it has been 68 years since Winogradsky (1890) accomplished the first isolation of nitrifying bacteria, the methods employed have not been improved significantly, and pure cultures remain incredibly difficult to obtain. The literature records numerous attempts to secure Nitrosomonas in pure culture. Nevertheless, the organism has seldom been isolated by dilution techniques. In the relat...
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A mineral medium for studying the growth of Nitrosomonas europaea was developed and examined. The medium was defined in terms of chemical speciation by using chemical equilibrium computer models. The medium significantly increased the metabolic activity of the organisms compared with previously developed media, yielding a specific growth rate as high as 3.0 day (generation time, 5.5 h). The spe...
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The purpose of this study was to measure the chlorine and monochloramine inactivation kinetics of Nitrosomonas europaea at 21 degrees C in the presence and absence of particles. The inactivation kinetics rates were compared with those obtained with Escherichia coli O157:H7. The results show that, in pure water, the use of free chlorine produced 4 log10 of N. europaea inactivation at a CT value ...
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عنوان ژورنال:
- Journal of general microbiology
دوره 4 2 شماره
صفحات -
تاریخ انتشار 1950